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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
2002 1
2004 1
2005 2
2006 1
2007 2
2008 5
2009 4
2010 5
2011 9
2012 6
2013 10
2014 15
2015 21
2016 16
2017 23
2018 30
2019 36
2020 32
2021 41
2022 41
2023 24
2024 10

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290 results

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Page 1
Twice-walk strategy based on three-dimensional DNA walking machine driven by duplex-specific nuclease.
Lei S, Liu Z, Zhang Q, Li Z, Li G, Zou L, Chai G, Mao J, Ye B, Zhang J. Lei S, et al. Chem Commun (Camb). 2024 Apr 2;60(28):3778-3781. doi: 10.1039/d3cc06058f. Chem Commun (Camb). 2024. PMID: 38494893
A twice-walk strategy based on a three-dimensional (3D) cleat-equipped DNA walking machine with a high signal amplification efficiency was investigated for ultrasensitive detection of miRNA. Impressively, addition of duplex-specific nuclease (DSN) just once d …
A twice-walk strategy based on a three-dimensional (3D) cleat-equipped DNA walking machine with a high signal amplification efficiency was i …
An integrated liposome-based microfluidic strategy for rapid colorimetric analysis: A case study of microRNA-21 detection.
Zeng X, Wang L, Liu C, Zhang J, Shi HW, Shen W, Kong D, Huang C, Lee HK, Tang S. Zeng X, et al. Talanta. 2024 May 15;272:125838. doi: 10.1016/j.talanta.2024.125838. Epub 2024 Feb 27. Talanta. 2024. PMID: 38430866
In the presence of miRNA-21, the DNA strand modified on the surface of Au nanoparticles hybridized with the target to form double-stranded products and was cleaved by duplex-specific nuclease (DSN) enzyme, causing the liposomes to be re-released. ...
In the presence of miRNA-21, the DNA strand modified on the surface of Au nanoparticles hybridized with the target to form double-stranded p …
Multiplexed discrimination of SARS-CoV-2 variants via duplex-specific nuclease combined MALDI-TOF MS.
Han G, Deng W, Lyu Q, Ma Q, Qiao L. Han G, et al. Anal Bioanal Chem. 2024 Mar;416(8):1833-1842. doi: 10.1007/s00216-024-05202-2. Epub 2024 Feb 17. Anal Bioanal Chem. 2024. PMID: 38367041
Consequently, assays capable of rapidly and simultaneously identifying multiple SARS-CoV-2 variants are essential for large-scale applications that aim to monitor the evolution of the virus. In this work, we propose a method combining duplex-specific nuclease …
Consequently, assays capable of rapidly and simultaneously identifying multiple SARS-CoV-2 variants are essential for large-scale applicatio …
Rigidifying AIEgens in covalent organic framework nanosheets for electrochemiluminescence enhancement: TABE-PZ-CON as a novel emitter for microRNA-21 detection.
Zhang JL, Wang TT, Liang WB, Yuan R, Xiao DR. Zhang JL, et al. Anal Chim Acta. 2024 Mar 22;1295:342321. doi: 10.1016/j.aca.2024.342321. Epub 2024 Feb 1. Anal Chim Acta. 2024. PMID: 38355235
Bearing in mind the exceptional ECL performance of TABE-PZ-CON, it was utilized as a high-efficient ECL indicator in combination with the DNA walker and duplex-specific nuclease-assisted target recycling amplification strategies to design an "off-on" ECL bios …
Bearing in mind the exceptional ECL performance of TABE-PZ-CON, it was utilized as a high-efficient ECL indicator in combination with the DN …
"DSN-mismatched CRISPR″sensor for highly selective and sensitive detection of under-expressed miR-let-7a.
Qin H, Chen Z, Zuo F, Cao R, Wang F, Wu H, Wang S, Xie Y, Ding S, Min X, Duan X. Qin H, et al. Anal Chim Acta. 2024 Mar 22;1295:342273. doi: 10.1016/j.aca.2024.342273. Epub 2024 Jan 27. Anal Chim Acta. 2024. PMID: 38355234 Free article.
Here, we describe a novel fluorescent biosensor for the highly selective and sensitive detection of miR-let-7a constructed by combining miRNA screening assisted by a duplex-specific nuclease (DSN) with CRISPR-Cas12a system signal amplification. ...
Here, we describe a novel fluorescent biosensor for the highly selective and sensitive detection of miR-let-7a constructed by combining miRN …
CRISPR-Cas12a based target recognition initiated duplex-specific nuclease enhanced fluorescence and colorimetric analysis of cell-free DNA (cfDNA).
Zhao C, Yang Z, Hu T, Liu J, Zhao Y, Leng D, Yang K, An G. Zhao C, et al. Talanta. 2024 May 1;271:125717. doi: 10.1016/j.talanta.2024.125717. Epub 2024 Jan 24. Talanta. 2024. PMID: 38281430
To detect cfDNA, researchers have created a CRISPR-Cas12a-based nucleic acid amplification-free fluorescent biosensor that uses a combination of fluorescence and colorimetric signaling improved by duplex-specific nuclease (DSN). DSN-assisted signal recycling …
To detect cfDNA, researchers have created a CRISPR-Cas12a-based nucleic acid amplification-free fluorescent biosensor that uses a combinatio …
A simultaneous strategy with multiple-signal amplification and self-calibration for ultrasensitive assay of miRNA-21 based on 3D MNPs-IL-rGO-AuNPs.
Yin M, Jiao J, Lu L, Hu B, Xue L, Dai F, Wang X, Wang Z, Wang T, Chen Q. Yin M, et al. Biosens Bioelectron. 2024 Apr 1;249:116009. doi: 10.1016/j.bios.2024.116009. Epub 2024 Jan 5. Biosens Bioelectron. 2024. PMID: 38199082
Herein, a self-calibration biosensing platform based on 3D novel MNPs-IL-rGO-AuNPs nanocomposites was successfully established for the ultrasensitive detection of miRNA-21. Duplex-specific nuclease (DSN) was introduced to recognize perfectly matched duplexes …
Herein, a self-calibration biosensing platform based on 3D novel MNPs-IL-rGO-AuNPs nanocomposites was successfully established for the ultra …
Profiling of RNA editing events in plant organellar transcriptomes with high-throughput sequencing.
Liu K, Xie B, Peng L, Wu Q, Hu J. Liu K, et al. Plant J. 2024 Apr;118(2):345-357. doi: 10.1111/tpj.16607. Epub 2023 Dec 27. Plant J. 2024. PMID: 38149801
However, the lack of commercial kits to remove rRNAs limits the usage of this method, especially for non-model plant species. DSN-seq is a transcriptome sequencing method utilizing duplex-specific nuclease (DSN) to degrade highly abundant cDNA species especia …
However, the lack of commercial kits to remove rRNAs limits the usage of this method, especially for non-model plant species. DSN-seq is a t …
An "off-on-off" type electrochemical biosensor for detecting multiple biomarkers with DNAzyme-mediated entropy-driven catalytic and DSN enzyme-assisted recycling amplification.
Yang H, Dong Q, Xu D, Feng X, He P, Song W, Zhou H. Yang H, et al. Anal Chim Acta. 2023 Dec 1;1283:341978. doi: 10.1016/j.aca.2023.341978. Epub 2023 Oct 28. Anal Chim Acta. 2023. PMID: 37977795
In the presence of microRNA 21(miRNA-21), the DNA/RNA heteroduplexes were formed and cleaved by duplex-specific nuclease (DSN) with recovery the target miRNA-21, causing the current suppression in an "on-off" state. ...
In the presence of microRNA 21(miRNA-21), the DNA/RNA heteroduplexes were formed and cleaved by duplex-specific nuclease …
290 results